Pogorzelska · 2024 · Scientific reports
Proliferation and metastasis markers in a mouse breast cancer model
How do low concentrations of sulforaphane affect cell proliferation and migration in models of triple-negative breast cancer?
Animal and cell · breast cancer mechanism experiment
Key findings
Tumor tissue and lung metastases in mice
The tumor cell-division marker was significantly lower, at 1.53 in the treated group versus 2.15 in controls. Lung metastatic nodules averaged 23 versus 34, but the between-group difference was not significant.
Cell migration by culture method
Inhibition of cell migration at the lower concentration was observed in cells derived from 3D spheroids. A higher concentration was needed to inhibit migration in 2D cultures.
Lung Metastatic Nodules · Mouse Model
Mean nodules per mouse
The between-group difference was not statistically significant
4T1 model · 8 mice per group
Read the original paper and sources ↓View values and units
| Group | Value |
|---|---|
| Control | 34 |
| SFN group | 23 |
Mitotic Index · Mouse Model
Mean tumor tissue mitotic index
Lower values indicate less cell division · significant difference observed
SFN 0.026 mg/kg · intraperitoneal injection
Read the original paper and sources ↓View values and units
| Group | Value | Standard deviation |
|---|---|---|
| Control | 2.15 | 0.13 |
| SFN group | 1.53 | 0.57 |
Study design
Sixteen female mice implanted with 4T1 tumors were compared in groups of eight. SFN 0.026mg/kg was injected intraperitoneally on days 10, 16, and 23; tissues were analyzed on day 28. Parallel 2D and 3D cell experiments were also conducted.
- Participants
- Mouse breast cancer model · eight per group
tumor tissue and lung metastases assessed - Intake and observation
- Sulforaphane 0.026mg/kg
intraperitoneal injection on days 10, 16, and 23 - Measures
- Lung metastatic nodules · cell-division marker
Study context
The mouse injection experiment was conducted alongside cell experiments. The tumor cell-division marker decreased significantly, while the difference in the number of lung metastatic nodules was not significant.
Original paper and sources
Antitumor and antimetastatic effects of dietary sulforaphane in a triple-negative breast cancer models.
- Journal
- Scientific reports · 2024 · 14(1): 16016
- First published
- 2024-07-11